Minimal Glucose Agar Settle Plate

Minimal Glucose Agar is used for the microbial mutagenicity Ames test. It is a bacterial bioassay performed in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. The principle is to detect point mutations using amino acid requiring strains. The Minimal Glucose Agar plate is taken as a bottom agar for the mutagenicity assay and includes the minimum ingredients for bacterial growth. Phosphates act as the buffering system. Glucose and Citrate are the carbon sources to produce energy. Magnesium is a cofactor for many metabolic reactions

Minimal Glucose Agar for Ames test is used to identify the revert mutations which are present in strains. It can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, reagents, cosmetics, wastewater, pesticides, and other substances which are easily solubilized in a liquid suspension.