biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
23IIC3, monoclonal
species reactivity
monkey, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL4A2(1284)
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General description
170 kDa
Immunogen
人羊膜IV型胶原蛋白
Application
使用经验证可用于ELISA、IH(P)& WB的该抗IV型胶原蛋白α 2链抗体(克隆23IIC3)检测IIV型胶原蛋白α 2链。
免疫组化:丙酮固定、冷冻组织建议采用1:500。 在1%胰蛋白酶处理(20分钟,室温)后,在福尔马林固定的石蜡切片(1:100)上有微弱的反应性。 或者,4% PFA 固定、HEIR处理的组织在1:200稀释度下对猴有效(Qin, 2003)。
蛋白质印迹:1:500, 170 kDa.
1:200 的ELISA
最佳工作稀释度必须由最终用户进行确定。
蛋白质印迹:1:500, 170 kDa.
1:200 的ELISA
最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
识别人IV型胶原蛋白α-2链。
Physical form
未纯化
液体。
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
Analysis Note
对照
阳性对照:肾脏、肌肉、肌腱、脾脏组织
阴性对照:神经元/神经胶质
阳性对照:肾脏、肌肉、肌腱、脾脏组织
阴性对照:神经元/神经胶质
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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