MAB3878
抗-MyoD1抗体
clone 5.2F, Chemicon®, from mouse
别名:
Anti-MYF3, Anti-MYOD, Anti-MYODRIF, Anti-PUM, Anti-bHLHc1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5.2F, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
8
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5.2F, monoclonal
species reactivity
rat, mouse, chicken, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYOD1(4654)
General description
约45 kDa
Immunogen
来自小鼠MyoD1的重组蛋白。
Application
抗MyoD1抗体是一种用于检测MyoD1的高质量小鼠单克隆抗体，&已在ICC、IHC、IP & WB中进行了验证。
蛋白质印迹：1 μg/mL。与~45 kD蛋白反应。
针对冷冻和福尔马林固定、石蜡包埋组织的免疫组织化学：2-4 μg/mL。
免疫细胞化学
免疫沉淀：2 μg/mg蛋白裂解物
最佳工作稀释度必须由最终用户确定。
针对冷冻和福尔马林固定、石蜡包埋组织的免疫组织化学：2-4 μg/mL。
免疫细胞化学
免疫沉淀：2 μg/mg蛋白裂解物
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别MyoD1。该表位位于小鼠MyoD1蛋白N末端的3-56位氨基酸。
Physical form
形式：纯化
Analysis Note
对照
胎儿肌肉，横纹肌肉瘤
胎儿肌肉，横纹肌肉瘤
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
Francesca Megiorni et al.
Journal of hematology & oncology, 10(1), 161-161 (2017-10-08)
EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR
Mark A Gillespie et al.
The Journal of cell biology, 187(7), 991-1005 (2009-12-23)
The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated significantly fewer myofibers than wild-type muscle. Notably
L V Renna et al.
European journal of histochemistry : EJH, 58(4), 2444-2444 (2015-01-13)
Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are multisystemic disorders linked to two different genetic loci and characterized by several features including myotonia, muscle weakness and atrophy, cardiac dysfunctions, cataracts and insulin-resistance. In both forms, expanded nucleotide sequences
Junmin Lee et al.
Biomaterials, 69, 174-183 (2015-08-19)
Cells sense and transduce the chemical and mechanical properties of their microenvironment through cell surface integrin receptors. Traction stress exerted by cells on the extracellular matrix mediates focal adhesion stabilization and regulation of the cytoskeleton for directing biological activity. Understanding
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