产品名称
抗生长相关蛋白-43(GAP-43)抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse (high degree), rat, bovine, chicken, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GAP43(2596)
Physical form
形式:纯化
在含有0.01%叠氮化钠作为防腐剂的PBS的缓冲液中的纯化兔多克隆抗体。
Analysis Note
对照
已进行脊神经结扎的大鼠背根神经节组织,培养神经元。
已进行脊神经结扎的大鼠背根神经节组织,培养神经元。
通过蛋白质印迹对小鼠脑裂解物进行常规评估。
蛋白质印迹分析:该批次的1:500的稀释液在10 μg小鼠脑裂解物中检测到生长相关蛋白43。
蛋白质印迹分析:该批次的1:500的稀释液在10 μg小鼠脑裂解物中检测到生长相关蛋白43。
Application
免疫组化:
先前批次的1:1,000稀释液用于4%多聚甲醛/PBS固定的组织切片或3%多聚甲醛/0.1%戊二醛/PBS固定的组织切片。
免疫细胞化学:
将先前批次的1:1,000稀释液用于培养物中的海马神经元和NGF刺激的PC12细胞。
免疫沉淀:
使用先前批次的1:100稀释液。
ELISA:
使用先前批次的1:2,000稀释液。
先前批次的1:1,000稀释液用于4%多聚甲醛/PBS固定的组织切片或3%多聚甲醛/0.1%戊二醛/PBS固定的组织切片。
免疫细胞化学:
将先前批次的1:1,000稀释液用于培养物中的海马神经元和NGF刺激的PC12细胞。
免疫沉淀:
使用先前批次的1:100稀释液。
ELISA:
使用先前批次的1:2,000稀释液。
抗生长相关蛋白-43(GAP-43)抗体是用于ELISA、IC、IH、IP &WB的针对生长相关蛋白-43(GAP-43)的抗体。
Biochem/physiol Actions
与大鼠,人,牛和鸡的反应程度较小。 其他物种尚未经过测试。
识别生长相关蛋白-43(GAP-43)。
General description
GAP-43是分化神经元的常见标志物,在轴突生长过程中通过发育或再生神经元以升高的水平表达。尽管GAP-43是与轴突生长锥(和突触)的细胞质表面相关的完整膜蛋白,但树突状生长锥中却不存在。在成人大脑中,GAP-43在认为发生记忆形成的突触前区域(例如额叶皮层,边缘系统和海马)以高浓度发现。PKC(人,小鼠,大鼠和牛GAP-43上的Ser41或鸡和非洲爪蟾GAP-43上的Ser42)对GAP-43的磷酸化与某些形式的突触可塑性特别相关。
23.6kda
Immunogen
重组大鼠GAP-43(完整序列)。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Preparation Note
自收到之日起,以未稀释的等分试样可于-20ºC条件下稳定保存6个月。
应避免反复冻/融循环。在运输过程中,少量产品有时会夹在产品瓶的密封中。 对于体积为200µL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器’盖中的所有液体。
应避免反复冻/融循环。在运输过程中,少量产品有时会夹在产品瓶的密封中。 对于体积为200µL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器’盖中的所有液体。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
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