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Merck
CN

ABN26

抗iNOS / NOS II抗体,NT

from rabbit, purified by affinity chromatography

别名:

Nitric oxide synthase, inducible, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, human
Citations:
19
Technique(s):
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
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产品名称

抗iNOS / NOS II抗体,NT, from rabbit, purified by affinity chromatography

conjugate

unconjugated

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

Gene Information

mouse ... Nos2(18126)

Application

使用经过验证可用于WB、IP、IH(P)的抗iNOS/NOS II抗体NT检测iNOS/NOS II。
免疫组化分析: 一个代表性批次产品以1:50-200的稀释度在恶性人肺组织中检测到iNOS/NOS II。

免疫沉淀分析: 10 µg该抗体从IFNγ/LPS处理的RAW264.7细胞裂解物中,对iNOS/NOSII进行了免疫沉淀。
研究子类别
氧化应激
研究类别
神经科学

Analysis Note

对照
未经处理和经IFNgamma/LPS处理的RAW264.7细胞裂解物
已通过蛋白质印迹在未经处理和经IFNgamma/LPS处理的RAW264.7细胞裂解物中进行了评估。

蛋白质印迹分析:0.5 µg/mL该抗体在10 µg未经处理和经IFNgamma/LPS处理的RAW264.7细胞裂解物中检测到iNOS/NOS II。

Biochem/physiol Actions

其他同源性:大鼠(85%序列同源性)。
该抗体可识别iNOS/NOS II的N端。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

一氧化氮(NO)是一种无机气态自由基,可在细胞之间传递多种信息。血管松弛、神经传递和细胞毒性都可以通过细胞对NO的反应来增强。NO的产生是由一氧化氮合酶(NOS)家族的成员介导的。NOS可催化L-精氨酸的氧化,生成L-瓜氨酸和NO。两种组成型亚型、大脑或神经元NOS(b或nNOS,I型) &、内皮细胞NOS(eNOS,III型)和一种诱导型亚型(iNOS,II型)已被克隆。细胞因子,例如干扰素-γ(IFN)、肿瘤坏死因子(TNF)、白介素-1和-2、以及脂多糖(LPS),会导致iNOS mRNA、蛋白质和活性水平的增加。蛋白激酶C刺激剂对iNOS活性具有相同的作用。人iNOS受钙/钙调蛋白的调节(与小鼠NOS2相反)。
观测值〜125 kDa

Immunogen

带有GST标签的重组蛋白,对应于小鼠iNOS/NOS II的N端。
表位:N端

Other Notes

替代品:06-573
浓度:请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

Preparation Note

自发运之日起,在 2-8°C 条件下可稳定保存1年

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分析证书(COA)

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Francesco Litta et al.
Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland, 24(12), 1567-1575 (2022-08-03)
The aetiology of cryptoglandular anal fistula (AF) is poorly understood. Evidence suggests that persistence and/or recurrence of the disease is more related to inflammatory than infectious factors. The aim of this study was to investigate the immune profile of cryptoglandular
Francisco J Salguero et al.
Parasites & vectors, 11(1), 73-73 (2018-02-02)
Visceral leishmaniasis (VL) is a neglected tropical disease (NTD), caused by the intracellular protozoan parasites Leishmania donovani and Leishmania infantum. Symptomatic VL is considered fatal when left untreated. At present, there is no effective vaccine licensed for human use and
Guillem Herrera-Torres et al.
Animals : an open access journal from MDPI, 14(12) (2024-06-27)
Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since the current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed to design effective
Kosaku Okuda et al.
Nature communications, 14(1), 621-621 (2023-02-05)
DNA methyltransferases (DNMTs) catalyze methylation at the C5 position of cytosine with S-adenosyl-L-methionine. Methylation regulates gene expression, serving a variety of physiological and pathophysiological roles. The chemical mechanisms regulating DNMT enzymatic activity, however, are not fully elucidated. Here, we show
Delphine Séhédic et al.
Theranostics, 7(18), 4517-4536 (2017-11-22)
Gold standard beam radiation for glioblastoma (GBM) treatment is challenged by resistance phenomena occurring in cellular populations well prepared to survive or to repair damage caused by radiation. Among signals that have been linked with radio-resistance, the SDF1/CXCR4 axis, associated

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