Anti-phospho Glucocorticoid Receptor (Ser134) Antibody

Glucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes that control development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can represses gene transcriptions by blocking the nuclear translocation of transcription factors. BDNF-stimulated TrkB activation is shown to induce GR phosphorylation at Ser155 and Ser287 in primary rat cortical neurons (equivalent to human GR Ser134 and Ser267), resulting in enhanced GR transcription activity. GR is composed of an N-terminal modulating domain (a.a. 1-420), a DNA-binding domain (a.a. 421-486), and a C-terminal ligand-binding domain (a.a. 528-777).

The target phosphorylation site recognized by this polyclonal antibody is present in all 9 human GR spliced isoforms (Ser108 of isoforms Alpha-B and Beta-B; Ser134 of all the other isoforms) and in both rat GR spliced isoforms (Ser155 of isoform A and Ser128 of isoform B) reported by UniProt (P04150 and P06536, respectively).

Epitope: pSer134.

Linear peptide corresponding to a human glucocorticoid receptor sequence containing phosphorylated Ser134.

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Hormones & Receptors

Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of endogenous GR in primary rat cortical neurons (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of exogenously expressed wild-type, but not S155A mutant, rat GR in transfected HEK293TrkB cells (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).

Evaluated by Western Blotting in cell lysate from HEK293 expressing FLAG-tagged human glucocorticoid receptor.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Ser134 phosphorylation of FLAG-tagged human glucocorticoid receptor in 10 ug lysate from BDNF-treated HEK293 transfectants.

~85 kDa observed. 87.56 kDa (Rat isoforms A), 84.83 kDa (Rat isoforms B), 85.66 kDa (Human isoform Alpha; Alpha-A), 81.51 kDa (Human isoform Beta; Beta-A), 85.82 kDa (Human isoform Alpha-2; Gamma), 81.67 kDa (Human isoform Beta-2), 64.75 kDa (Human isoform GR-A alpha), 60.60 kDa (Human isoform GR-A beta), 82.85 kDa (Human isoform Alpha-B), 78.70 kDa (Human isoform Beta-B), 82.90 kDa (Human isoform 10; hGRDelta313-338) calculated. Uncharacterized band(s) may appear in some lysates.

Affinity purified

Purified rabbit polyclonal antibody in Tris-buffered saline (pH 8.0) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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