Anti-Endoglin Antibody, Extracellular, clone MJ7/18

Endoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor.

Inflamed mouse skin (Ge, AZ et al, 1994).

Flow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.

Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.

Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.

Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin.

Specifically recognizes mouse CD105, known as endoglin.

Format: Purified

Protein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives.

Maintain at 2-8°C for up to 12 months from date of receipt.

Control
POSITIVE CONTROL: mouse vascular endothelial cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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