biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
V284, monoclonal, VLN01, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (1 µg/mL, chemiluminescence)
Immunoprecipitation (2 µg/mL lysate)
Paraffin Sections (4-8 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
General description
该抗-纽蛋白小鼠mAb（V284或VLN01）经验证可用于免疫印迹，免疫沉淀，石蜡切片以检测纽蛋白。
蛋白G纯化的小鼠单克隆抗体，通过用指定的免疫原免疫BALB/c小鼠，并将脾细胞与小鼠骨髓瘤SP2/0细胞融合而产生。识别~130（纽蛋白）和~150 kDa（间纽蛋白）蛋白。
识别皮肤组织中的~130（纽蛋白）和~150 kDa（间纽蛋白）蛋白。
Immunogen
人
半纯化人纽蛋白
Application
免疫印迹法（1 g/mL，化学发光法）
免疫沉淀（2 g/mL裂解物）
石蜡切片（4-8 g/ml，需要加热预处理）
Packaging
请参考特定浓度批号的标签。
Physical form
在10 mM PBS，0.2%BSA，pH值7.4中。
Analysis Note
阳性对照
皮肤细胞或组织
皮肤细胞或组织
阴性对照
正常小鼠血清
正常小鼠血清
Other Notes
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J.1990 Cell Motil.Cytoskeleton16, 1.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J.1990 Cell Motil.Cytoskeleton16, 1.
该抗体可用于研究纽蛋白在血小板活化中的作用。当用于免疫组化时，纽蛋白（Ab-1）表现出膜和细胞质染色。对于福尔马林固定石蜡切片的染色，在10 mM柠檬酸盐缓冲液（pH 6.0）中煮沸10-20分钟，然后在室温下冷却20分钟。应滴定抗体，以在单个系统中获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
未找到合适的产品？
试试我们的产品选型工具.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Nitric Oxide Circumvents Virus-Mediated Metabolic Regulation during Human Cytomegalovirus Infection.
Rebekah L Mokry et al.
mBio, 11(6) (2020-12-17)
Nitric oxide is a versatile and critical effector molecule that can modulate many cellular functions. Although recognized as a regulator of infections, the inhibitory mechanism of nitric oxide against human cytomegalovirus (HCMV) replication remains elusive. We demonstrate that nitric oxide
Mizuko Ikeda et al.
Critical care (London, England), 19, 332-332 (2015-09-19)
There is a sex difference in the risk of ischemic acute kidney injury (AKI), and estrogen mediates the protective effect of female sex. We previously demonstrated that preprocedural chronic restoration of physiologic estrogen to ovariectomized female mice ameliorated AKI after
Steve Seltzsam et al.
Translational oncology, 12(3), 417-425 (2018-12-17)
Human papillomavirus (HPV) associated squamous cell carcinomas of the head and neck region (HPV+ HNSCCs) harbor diverging biological features as compared to classical noxa-induced (HPV-) HNSCC. One striking difference between subtypes is that the tumor suppressor gene TP53 is usually
Harikrishnareddy Paluvai et al.
Molecular oncology, 12(12), 2165-2181 (2018-10-14)
Expression of the class IIa HDACs is frequently altered in different human cancers. In mouse models these transcriptional repressors can trigger transformation, acting as bona fide oncogenes. Whether class IIa HDACs also exhibit transforming activities in human cells is currently
Christian Redeker et al.
PloS one, 8(10), e79050-e79050 (2013-10-30)
The Notch signaling pathway mediates the direct communication between adjacent cells and regulates multiple developmental processes. Interaction of the Notch receptor with its ligands induces the liberation of the intracellular portion of Notch (NICD) referred to as regulated intramembraneous proteolysis
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CP74-100UG | 04055977221022 |
| P1484-.2ML | 04061837868269 |
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持