MABC959
Anti-CD1d Antibody, clone WTH-1
clone WTH-1, from mouse
别名:
Antigen-presenting glycoprotein CD1d, CD1d
选择尺寸
关于此项目
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
WTH-1, monoclonal
species reactivity
mouse, rat
technique(s)
activity assay: suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Cd1D(110290701)
rat ... Cd1D(104874051)
General description
Application
Flow Cytometry Analysis: A representative lot detected the surface expression of CD1d on isolated thymocytes from rats and mice, as well as on Raji transfectants expressing murine or rat CD1d (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD1d from isolated mouse and rat thymocytes, as well as exogenously expressed murine and rat CD1d from transfected Raji cells (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Immunohistochemistry Analysis: A representative lot detected CD1d immunoreactivity using frozen tissue sections from rats and mice (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Activity Assay: A representative lot inhibited CD1d antigen presentation cells from activating type I NK cells using total splenocytes from rats and mice. Clone WTH-1 also blocked mCD1d1-transduced LBB cells from activating co-cultured VIII24.1 murine type II NKT cells, while WTH-1 enhanced XV19.2 murine type II NKT cell activation by mCD1d1-transduced Raji cells (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Application Note: Clone WTH-1 (Cat. No. MABC959) and WTH-2 (Cat. No. MABC960) gave inferior IHC staining in mouse than in rat organs, especially in non-lymphatic tissues. In addition, clone WTH-2 produced a much lower signal in mice than mAb WTH-1. To detect low-grade expression, mouse non-lymphatic organs should be stained using a highly sensitive tyramide amplification procedure with inclusion of C57BL/6 CD1d2/2 mouse organs as negative controls (Monzon-Casanova, E., et al. (2010). PLoS One. 5(9): e13089).
Physical form
Analysis Note
Flow Cytometry Analysis: 1.0 µg of this antibody detected CD1d in rat thymocytes and splenocytes.
Other Notes
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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