生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
hT2H21F11, monoclonal
种属反应性
human, mouse
技术
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TET2(54790)
一般描述
甲基胞嘧啶双加氧酶TET2催化甲基胞嘧啶（5mC）向5-羟甲基胞嘧啶（5hmC）的转化。目前尚不清楚5-羟甲基胞嘧啶的功能，但它可能影响染色质结构，或充当胞嘧啶去甲基化的中间成分。TET2经常在骨髓增生性疾病(MPD)或骨髓增生性肿瘤(MPN)和全身性肥大细胞增多症中发生突变。TET2疾病还会引起真性红细胞增多症(PV)和骨髓增生异常综合症(MDS)。
观测分子量〜260 kDa。该蛋白的计算分子量为224 kDa，但可在约260 kDa处观察到
免疫原
对应于人TET2 N末端的重组蛋白。
应用
使用该小鼠单克隆抗体（抗TET2抗体，克隆hT2H 21F11）检测Tet2，该抗体经验证可用于染色质IP（ChIP）和蛋白质印迹& IP。
蛋白质印迹分析：1.0 µg/mL的代表性批次在10 µg TF-1细胞裂解物中检测到TET2。
免疫沉淀分析：5 µg的代表性批次在250 µg HL60 RIPA细胞裂解物中免疫沉淀TET2。
免疫沉淀分析：5 µg的代表性批次在250 µg HL60 RIPA细胞裂解物中免疫沉淀TET2。
生化/生理作用
该抗体识别TET2的N末端
外形
形式：纯化
分析说明
对照
MOLT-4细胞裂解物
MOLT-4细胞裂解物
通过蛋白质印迹法在MOLT-4细胞裂解物中进行评价。
蛋白质印迹分析：1.0 µg/mL的该抗体在10µg MOLT-4细胞裂解物中检测到TET2。
蛋白质印迹分析：1.0 µg/mL的该抗体在10µg MOLT-4细胞裂解物中检测到TET2。
其他说明
浓度：关于批次特定浓度请参见检验报告。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Lei-Lei Chen et al.
Science advances, 6(38) (2020-09-20)
TET2 DNA dioxygenase is frequently mutated in human hematopoietic malignancies and functionally inactivated in many solid tumors through a nonmutational mechanism. We recently found that TET2 mediates the interferon-JAK-STAT pathway to stimulate chemokine expression and tumor infiltration of lymphocytes (TILs).
Zihang Zeng et al.
Redox biology, 67, 102916-102916 (2023-10-10)
Chronic obstructive pulmonary disease (COPD) is a significant global cause of morbidity and mortality currently. Long-term exposure of cigarette smoke (CS) inducing persistent inflammation, small airway remodeling and emphysematous lung are the distinguishing features of COPD. Ferroptosis, occurred in lung
Roser Vento-Tormo et al.
The Journal of allergy and clinical immunology, 139(1), 202-211 (2016-07-11)
Inflammasomes are cytosolic multiprotein complexes in macrophages. They assemble after infection- or stress-associated stimuli, activating both caspase-1-mediated inflammatory cytokine secretion and pyroptosis. Increased inflammasome activity resulting from gene mutations is related to monogenic autoinflammatory syndromes. However, variable penetrance among patients
Qi Wang et al.
mBio, 10(4) (2019-08-23)
HIV-1 Vpr enhances viral replication in human macrophages via multiple mechanisms that are not clearly defined. It does not affect HIV-1 virion production during the first round of infection. We have recently discovered that Vpr targets the DNA demethylase TET2
Lijuan Luo et al.
iScience, 27(3), 109252-109252 (2024-03-05)
DNA demethylase TET2 was related with lung function. However, the precise role of TET2 in cigarette smoke (CS)-induced apoptosis of airway epithelium cells, and the mechanisms involved, have yet to be elucidated. Here, we showed that CS decreased TET2 protein
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