Anti-HGPRT Antibody, clone 13H11.1

Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8; UniProt P00492; also known as HGPRT, HGPRTase) is encoded by the HPRT1 (also known as HGPRT, HPRT) gene (Gene ID 3251) in human. HPRT is a purine salvage enzyme that catalyzes the condensation of 5-phosphoribosyl 1-pyrophosphate (PRPP) and hypoxanthine or guanine to form inosine 5-monophosphate (IMP) and guanosine 5-monophosphate (GMP), respectively. PRPP is an essential compound for the de novo pathway of purine synthesis as well as the synthesis of pyrimidine compounds. PRPP concentrations in erythrocyte are markedly increased in individuals with HPRT deficiency. Complete HPRT deficiency leads to Lesch–Nyhan disease (LND) characterized by uric acid overproduction, motor dysfunction, cognitive disability, and self-injurious behavior. Partial deficiency of HPRT activity causes Lesch–Nyhan variant (LNV) characterized by hyperuricemia and variable degree of neurological manifestations, depending on the residual enzyme activity.

~25 kDa observed

Epitope: Internal

GST-tagged human HGPRT recombinant fragment lacking N- and C-terminal regions.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-HGPRT, clone 13H11.1 is validated for use in Western Blotting for the detection of HGPRT.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HGPRT in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.