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Merck
CN

OP104L

Sigma-Aldrich

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

lyophilized, clone PAb1802, Calbiochem®

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UNSPSC代码:
12352203
NACRES:
NA.41

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生物来源

mouse

质量水平

100

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

PAb1802, monoclonal

表单

lyophilized

不包含

preservative

种属反应性

human, mouse

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

dilution

(Immunoblotting (2.5 µg/mL, chemiluminescence,
Immunoprecipitation (1 µg/reaction,
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended))

同位素/亚型

IgG1

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... TP53(7157)

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).

免疫原

Epitope: within amino acids 306-393 of human p53
Human
recombinant, human p53 protein

应用

Immunoblotting (2.5 µg/ml, chemiluminescence, see application references)

Immunoprecipitation (1 µg/reaction, see comments, see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

外形

Lyophilized from a volatile buffer, 100 µg BSA.

制备说明

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.

分析说明

Negative Control
SK-OV-3 cells
Positive Control
A431 or SK-BR-3 cells

其他说明

Levine, A.J. 1997. Cell88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol.17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J.15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA92, 9455.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Clarke, A.R., et al. 1994. Oncogene9, 1767.
Cho, Y., et al. 1994. Science265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Toxicity: Standard Handling (A)

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储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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