OP104L
Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)
lyophilized, clone PAb1802, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PAb1802, monoclonal
Species reactivity:
human, mouse
Application:
—
Citations:
1
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb1802, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (2.5 µg/mL, chemiluminescence,
Immunoprecipitation (1 µg/reaction,
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended))
isotype
IgG1
shipped in
ambient
Quality Level
Gene Information
human ... TP53(7157)
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).
Immunogen
Epitope: within amino acids 306-393 of human p53
Human
recombinant, human p53 protein
Application
Immunoblotting (2.5 µg/ml, chemiluminescence, see application references)
Immunoprecipitation (1 µg/reaction, see comments, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Immunoprecipitation (1 µg/reaction, see comments, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Physical form
Lyophilized from a volatile buffer, 100 µg BSA.
Preparation Note
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
Analysis Note
Negative Control
SK-OV-3 cells
SK-OV-3 cells
Positive Control
A431 or SK-BR-3 cells
A431 or SK-BR-3 cells
Other Notes
Levine, A.J. 1997. Cell88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol.17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J.15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA92, 9455.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Clarke, A.R., et al. 1994. Oncogene9, 1767.
Cho, Y., et al. 1994. Science265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Miller, S.D., et al. 1997. Mol. Cell. Biol.17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J.15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA92, 9455.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Clarke, A.R., et al. 1994. Oncogene9, 1767.
Cho, Y., et al. 1994. Science265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Kimberley J Botting et al.
Journal of the American Heart Association, 3(4) (2014-08-03)
Placental insufficiency is the leading cause of intrauterine growth restriction in the developed world and results in chronic hypoxemia in the fetus. Oxygen is essential for fetal heart development, but a hypoxemic environment in utero can permanently alter development of
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