CpG WIZ^™ E-Cadherin Amplification Kit

E-cadherin is a cell adhesion protein that suppresses tumor cell invasion and metastasis in tumor models. On mechanism regulating the expression of the E-cadherin gene is methylation of the promoter region. The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated) for E-cadherin are also included.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ E-cadherin Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

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