BioTracker^™ Putrescine Polyamine Green Fluorescent Live Cell Probe

red-brown solid

Store at -20 °C, protected from light

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Polyamines (putrescine, spermine, spermidine) play a variety of critical roles in the cell. Some of these processes include gene expression, cell proliferation and differentiation, and cellular stress management. Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal, ovarian, prostate, and pancreas. In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.

The relationship between polyamine systems and cancer---plus critical normal cell processes--- led to the creation of clickable polyamine derivatives which are innovative and novel tools for functional studies of the polyamine transport system (PTS). Putrescine and other important polyamine derivatives were conjugated to a BODIPY fluorophore through click chemistry.MW: 558.34 g/mol SPECTRAL PROPERTIESλex: 494 nmλem: 508 nm

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The BioTracker^™ Clickable Polyamine Putrescine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine transport with superior cellular uptake.Probe may be dissolved in DMSO to make a 10mM stock solution. 1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.

This putrescine derivative click-conjugated to BODIPY is optiized for the study of the polyamine transport system, which is exploited in diverse cancers.