form
solution
packaging
vial of 10 μmol
manufacturer/tradename
Roche 10427922001
storage temp.
−20°C
General description
N1-Methyl-Pseudo-UTP (N1-Methylpseudouridine-5′-triphosphate) is a modified nucleoside triphosphate where the N1 position of pseudouridine is methylated. This modification significantly influences RNA structure and function, particularly enhancing translation efficiency and stability
Application
Enhanced Translation Efficiency: N1-Methyl-Pseudo-UTP is used in the synthesis of mRNA to improve translation efficiency. The modified nucleotide can lead to increased protein yields in in vitro translation systems.Stability Studies: The incorporation of N1-Methyl-Pseudo-UTP into RNA allows researchers to study the effects of nucleotide modifications on RNA stability. This application is crucial for understanding how modifications impact RNA degradation rates and half-lives.Functional RNA Applications: The use of N1-Methyl-Pseudo-UTP in synthesizing functional RNA, such as ribozymes and RNA aptamers, can enhance their stability and functionality, making them more effective in various biological assays.Synthetic Biology: N1-Methyl-Pseudo-UTP is utilized in synthetic biology to create RNA molecules with tailored properties, enabling the design of RNA circuits and regulatory elements with improved performance.
Features and Benefits
- Improved Translation Efficiency: The methylation at the N1 position of pseudouridine enhances the interaction between mRNA and the ribosome, leading to more efficient translation and higher protein yields.
- Increased Stability: RNA transcripts synthesized with N1-Methyl-Pseudo-UTP exhibit greater resistance to degradation by nucleases, resulting in longer-lasting RNA in cellular environments.
- Versatile Applications: The modified nucleotide can be incorporated into various RNA synthesis protocols, making it suitable for a wide range of research applications.
- Enhanced Functional Performance: The use of N1-Methyl-Pseudo-UTP in functional RNA studies can improve the performance of RNA molecules, increasing their effectiveness in biological assays
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