sterility
sterile; 0.45 μm filtered
form
dry powder
shelf life
limited shelf life, expiry date on the label
technique(s)
microbiological culture: suitable
solubility
sterile water: 0.12 g/mL
application(s)
food and beverages
pharmaceutical
water monitoring, microbiology
shipped in
ambient
storage temp.
2-8°C
suitability
selective for bacteria: Clostridium perfringens
Quality Level
General description
C. perfringens Millichrome™ plus Supplement 2 is a selective media supplement for the detection and enumeration of C. perfringens. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. One vial (0.6 g) of the supplement is sufficient for 5L of final media. The prepared supplement is added to the melted C. perfringens Millichrome™ plus Agar Base at 45-50 °C.
Application
C. perfringens Millichrome™ plus Supplement 2 is a selective chromogenic media supplement intended for use in the qualitative detection and enumeration of C. perfringens from various samples.
Packaging
1 vial à 0.6g (with screw cap) is sufficient for 5L
Preparation Note
- Add 0.12 g of C. perfringens Millichrome™ plus supplement 2 in 1 mL of purified water.
- Filter to sterilize at 0.45 μm
- Add 1 mL/L of the supplement 2 solution to the melted base at 45/50 °C.
Analysis Note
The Clostridium perfringens is detected by a characteristic orange color after 24 h at an optimal growth temperature of 37°C, while Clostridium difficile forms blue to green colonies after incubation at the same temperature.
Legal Information
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
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商品
Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
相关内容
68075 C. perfringens Millichrome™ plus Supplement 2
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