A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line.

The hybrid cell line1.1B4 was formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802).

According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay. 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.

The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442)

Split sub-confluent cultures (70-80%) 1:2 to 1:6, i.e., seeding at 2-4 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approximately 20 hours. At confluence 10⁵ cells/cm² can be expected.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.