Merck
CN

10065

Sigma-Aldrich

α-淀粉酶 来源于米曲霉

powder, ~30 U/mg

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别名:
1,4-α-D-葡聚糖-葡萄糖水解酶
EC 号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

形式

powder

质量水平

specific activity

~30 U/mg

分子量

Mr ~51000

储存温度

2-8°C

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此商品
86247A9857A8220
specific activity

~30 U/mg

specific activity

~100 U/mg

specific activity

≥150 units/mg protein (biuret)

specific activity

≥800 FAU/g

mol wt

Mr ~51000

mol wt

-

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

一般描述

微生物淀粉酶是胞外酶,可用于多种工业应用,如面包和麦芽糖浆生产、酱油和豆面酱发酵等。从米曲霉中分离的α-淀粉酶的分子量为51kDa(沉降和扩散)和49kDa(凝胶过滤)。

应用

α-淀粉酶已被用作
  • 琼脂平板和羧甲基纤维素清除试验的对照酶,用于筛选淀粉水解的纤维素酶活性,
  • 以便探讨小麦淀粉在冷冻面团中
  • 抑制金黄色葡萄球菌生物膜的作用。

生化/生理作用

米曲霉α-淀粉酶(Ao α-淀粉酶)酶催化可溶性淀粉和相关底物中α-1,4糖苷键的水解。这些底物被分解并释放较短的低聚糖和α-极限糊精。

单位定义

1个酶活力单位(U)相当于在pH6.0和25℃下每分钟释放1μmol麦芽糖所需的酶量(淀粉符合Zulkowsky,货号85642,作为底物)。

其他说明

用于测定食物中的脂肪;应用于糖苷键的(选择性)水解/缩合

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险声明

危险分类

Acute Tox. 4 Oral - Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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S Wirsel et al.
Molecular microbiology, 3(1), 3-14 (1989-01-01)
We have cloned three genes (amy1, amy2 and amy3) encoding alpha-amylase in the filamentous fungus Aspergillus oryzae. The established overall sequences have a very high degree of homology, showing divergences mainly in the 3'-untranslated regions. The positions and the sequences
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