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11191

Histopaque^®-1119

Name: Histopaque<SUP>®</SUP>-1119
Brand: SIGMA

sterile-filtered, density: 1.119

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关于此项目

NACRES:

NA.75

UNSPSC Code:

12352207

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sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.119

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque^®-1119 是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成，调配的密度为1.119 g/mL，pH为8.8–9.0。

Application

Histopaque^®-1119适合与Histopaque-1077一起使用，因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。

Biochem/physiol Actions

该过程涉及在 Histopaque-1119 上使等体积的 Histopaque-1077 分层，形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心后，红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119 界面；而在上部血浆/Histopaque-1077 界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。

Features and Benefits

Histopaque^®-1119 有许多优势，比如：

可使活细胞极佳恢复
允许选择分离不同种类的血细胞
防止细胞变形
保持细胞活力
最小化外来细胞干扰
各批次性能可重复
经过过滤杀菌和内毒素检测
在 2-8°C 避光保护条件下可稳定保存至少2年

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

GHS05

signalword

Warning

hcodes

H290,H315,H319

pcodes

P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Identification of pneumococcal colonization determinants in the stringent response pathway facilitated by genomic diversity.

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BMC genomics, 16, 369-369 (2015-05-10)

Understanding genetic determinants of a microbial phenotype generally involves creating and comparing isogenic strains differing at the locus of interest, but the naturally existing genomic and phenotypic diversity of microbial populations has rarely been exploited. Here we report use of

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商品

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

默克密度梯度介质_细胞分离-默克生命科学

我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。

实验方案

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400^® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

全球贸易项目编号

货号	GTIN
251429-50G	04061838138613
251429-10G	04061838138606
11191-6X100ML	04061838260659
11191-100ML	04061838699305

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