无菌性
sterile-filtered
质量水平
保质期
Expiry date on the label.
杂质
endotoxin, tested
密度
1.119 g/mL
application(s)
hematology
histology
储存温度
2-8°C
一般描述
应用
将等体积的Histopaque-1077铺在 Histopaque-1119上，可以形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心时，红细胞被聚蔗糖聚集并迅速沉淀。粒细胞位于下部的Histopaque-1077/1119 界面，而淋巴细胞和其他单核细胞存在于上部的血浆/Histopaque-1077界面。它液适用于从鸡骨髓细胞悬浮液中提纯树突状细胞和从小鼠骨髓细胞悬浮液中提纯中性粒细胞。
特点和优势
- 可使活细胞极佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在 2-8°C 避光保护条件下可稳定保存至少2年
原理
法律信息
相关产品
警示用语：
Warning
危险分类
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
储存分类代码
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
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示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
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未找到您寻找的产品？
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
When using Product 11191, Histopaque®-1119, the mononuclear cell layers forms as expected, but the layer with the polymorphonuclear (eg, neutrophils) does not form properly. Why?
Technique is much more important with Histopaque® 1119. The published product insert calls for layering in the 1119 first, then adding a layer of 1077 and then layering the blood. If there is any mixing between the 1077 and the 1119, the procedure does not work properly. Customers can check for the integrity of their interface by checking for Schlieren optics. Any swirling or mixing should be evident when holding the tube up against a light source, such as a ceiling light or towards a window. If the interface was properly prepared, there should be a sharply demarcated line. If swirling is present, the user should start over. It is also important to use the gradient as soon as it is formed. There are no chemical differences between 1077 and 1119. They are the same product, only different densities. If left together for any length of time the two products will start diffusing together. When this happens the percent recovery becomes unacceptable. The gradients must be used immediately after preparation - without delay. Creating the gradient requires the steadiest of hands. Until you master the technique, limit the number of samples you attempt to process in one run. After gaining experience, the number of samples processed at one time can be increased.Some customers prefer to underlay. This protocol starts with 1077 in the test tube. Then a long metal cannula or a 9.5 in. Pasteur pipet is used to introduce the 1119 into the very bottom of the test tube. Care must be taken not to introduce any air bubbles. The air bubbles will destroy the interface. Load ~2.9 mls of 1119 into a 9.5 in. Pasteur pipette. With an electric pipet aid, add very slowly to the bottom of the tube. With this technique the Histopaque® 1077 is slowly pushed upwards and displaced by the Histopaque® 1119 When done properly very sharp gradients are possible. Electric pipet aids are useful when overlaying and underlaying Histopaque®. The release is dependent upon how slow or fast the dispensing button is applied. Regardless of the technique employed, it is imperative to introduce the Histopaque® slowly and smoothly. Users trying to do this with a 10 ml pipet hooked up to a blue rubber bulb are often going to experience problems. When dispensing, tilt the tube slightly, touch the pipet to the Histopaque® already placed in the centrifuge tube, draw the meniscus up the side of the tube 4 or 5 mm and then allow the Histopaque® to be released. No mixing should occur. If dispensed too quickly, the Histopaque®/blood will mix with the lower layer.When releasing the Histopaque® into the centrifuge tube it is beneficial to slant the tube at a 45 degree angle. The Histopaque® is then slowly released. When complete there should be an absolutely sharp, visible line at the interface between the Histopaque® 1077 and the Histopaque® 1119.It is imperative the blood and Histopaque® all be at room temperature. Having either the blood or Histopaque® cold could well cause the red cell contamination to increase and the cell recovery to become unacceptable.Another tip involves the measurement of the radius for your centrifuge. The measurement should be from the center of the spindle to the bottom of the centrifuge tube (when in the swung out position). Calculating the radius to the top or the middle of the centrifuge tube could also account for separation problems. It is debatable which method is correct - top, bottom or middle. Sigma uses the bottom of the centrifuge measurement. Some centrifuges automatically calculate rpm. Check the owner's manual for your centrifuge to determine how the rpm calculation is performed. Not all manufacuters use the method recommended by Sigma-Aldrich.If you are collecting your blood with a syringe, switch over to a vacuum tube. Use the freshest blood possible, but do allow the blood to reach room temperature.
Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
My question is not addressed here, how can I contact Technical Service for assistance?
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商品
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。
实验方案
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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