grade
Molecular Biology
product line
BioReagent
form
powder
quality
5× concentrate
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
color
white
pH
8.1-8.5
storage temp.
room temp
General description
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.
Application
TRIS borate – EDTA buffer substance is suitable for use as running buffer for agarose gel electrophoresis and nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.
Other Notes
The final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
新产品
此项目有
Detection of Chicken Meat Adulteration in Raw Hamburger Using
Polymerase Chain Reaction
Polymerase Chain Reaction
M. Mehdizadeh
Journal of Food Quality (2014)
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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