产品名称
氧钒核糖核苷复合物 溶液, BioReagent, Molecular Biology, 200 mM
Quality Level
grade
Molecular Biology
product line
BioReagent
form
liquid
concentration
200 mM
impurities
oxovanadium V
suitability
in accordance for ribonuclease inhibition test
shipped in
dry ice
storage temp.
−20°C
Application
氧钒核糖核苷复合物溶液可用于：
- 作为多核糖体裂解缓冲液和荧光原位杂交（FISH）缓冲液的成分
- 作为 RNase 抑制剂，从小鼠睾丸中分离出内源性 YTH 结构域家族蛋白 2（YTHDC2）
氧钒核糖核苷复合物（VDR）是过渡态类似物，可与许多 RNase 的活性位点结合，从而抑制其活性。由于 RNase 不会被该复合物共价修饰，因此必须在 RNA 提取和纯化的每个阶段使用 VDR。但是，VDR 还抑制 RNA 聚合酶和体外翻译，因此必须从 RNA 的最终制剂中去除 VDR。
General description
氧钒核糖核苷复合物是核糖核酸酶的低分子量抑制剂。
氧钒核糖核苷复合物溶液是核糖核酸酶（RNase）的抑制剂。在组织、基因组、RNA 和蛋白质样品中使用氧钒核糖核苷复合物可防止 RNase 降解 RNA。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Immunoprecipitation of mRNA-protein complexes
Peritz T, et al.
Nature Protocols, 1(2), 577-577 (2006)
Regulation of m 6 A Transcripts by the 3?? 5? RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline
Wojtas MN, et al.
Molecular Cell, 68(2), 374-387 (2017)
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Haimovich G and Gerst JE
Proceedings of the National Academy of Sciences of the USA, 8(21) (2018)
P A Takizawa et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(10), 5273-5278 (2000-05-03)
In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of
Wei Wang et al.
Nucleic acids research, 36(15), 4913-4928 (2008-07-26)
The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport
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