biological source
mouse
Quality Level
recombinant
expressed in mouse cell line
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
MAT3-4G10/8, monoclonal
form
PBS solution
mol wt
antigen ~300 kDa
species reactivity
human, mouse
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 μg/mL using HEK-293T total cel extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATM(472)
mouse ... Atm(11920)
General description
单克隆抗ATM(小鼠IgG1同种型)源自通过小鼠骨髓瘤细胞(NSO)融合产生的杂交瘤MAT3-4G10/8和用肽免疫的BALB/c小鼠脾细胞。
Immunogen
跨越小鼠ATM的1967-1988位的肽,在其NH2末端含有与KLH偶联的半胱氨酸。
Application
ATM单克隆抗体可用于免疫印迹、ELISA和免疫沉淀。
Biochem/physiol Actions
单克隆抗ATM抗体与小鼠和人ATM发生特异性反应。
抗共济失调毛细血管扩张突变(ATM)负责响应双链断裂(DSB)激活和稳定p53。ATM直接在Ser15上磷酸化p53,并同时激活其他激酶,这些激酶在其他位点磷酸化同一分子。此外,双分钟2(Hdm2)的人类同源物在Ser395上被ATM磷酸化,这种磷酸化抑制了Hdm2介导的p53降解。
Physical form
0.01 M磷酸盐缓冲液(pH 7.4,含有15 mM叠氮化钠)中的溶液
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
常规特殊物品
低风险生物材料
此项目有
相关内容
Instructions
ATM-dependent phosphorylation of Mdm2 on serine 395: role in p53 activation by DNA damage
Maya R, et al.
Genes & Development, 15(9), 1067-1067 (2001)
Phosphorylation of Hdmx mediates its Hdm2- and ATM-dependent degradation in response to DNA damage
Pereg Y, et al.
Proceedings of the National Academy of Sciences of the USA, 102(14), 5056-5061 (2005)
Marta Viana-Pereira et al.
PloS one, 6(5), e20588-e20588 (2011-06-04)
High grade gliomas (HGG) are one of the leading causes of cancer-related deaths in children, and there is increasing evidence that pediatric HGG may harbor distinct molecular characteristics compared to adult tumors. We have sought to clarify the role of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A1106-100UL | 04061837863387 |
| A1106-200UL | 04061837863394 |