D7295
脱氧核苷酸混合物，10mM
Molecular Biology Reagent
别名:
脱氧核苷酸混合物，10mM
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关于此项目
NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
biological source
synthetic (organic)
Quality Level
assay
≥99%
form
liquid
concentration
10 mM
color
colorless
solubility
H2O: soluble at 20 °C
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
核苷酸是有机分子，可作为核酸（如DNA和RNA）的单体或亚基。核苷酸是核酸的结构单元，由一个含氮碱基（嘌呤或嘧啶）、一个五碳糖（核糖或脱氧核糖）和至少一个磷酸基团组成。一个核苷与一个磷酸基团组合形成一个核苷酸。脱氧核苷酸混合物是一种便捷式预混dNTP溶液，以高质量分子生物学级水为溶剂，含有等摩尔（10mM）的UltraPure超纯dATP、dCTP、dGTP和TTP钠盐。1 µL足够进行一次标准50 µL PCR反应。
Application
dNTP混合物已用于：
- PCR扩增昆虫、 真菌、病毒、人类来源分离的基因组DNA
- 总RNA逆转录为cDNA
- 作为聚合酶链式反应(PCR)DNA扩增混合物组分
- 常规和长片段PCR
- 手动和自动DNA测序
- cDNA合成和标记反应
Features and Benefits
- 每种dNTP的纯度：最低99%
- 方便配制；每50 μL PCR使用1 μL
- 等摩尔量的各种dNTP可减少移液步骤
- 最大限度地降低PCR污染风险
- UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Ajay V Maker et al.
Journal of the American College of Surgeons, 228(5), 721-729 (2019-02-23)
Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay
Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains
Unal Egeli et al.
Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D7295-5ML | 04061838104953 |
| D7295-.5ML | 04061838104946 |
| D7295-.2ML | 04061838104939 |
| D7295-.25ML | 04061838056863 |
| D7295-20X.2ML | 04061838087355 |