sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
General description
ITS+3液体培养基补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠、亚油酸和牛血清白蛋白(BSA)的混合物。适合无血清细胞培养应用。
Application
ITS+3液体培养基补充剂(100×)作为培养基补充剂已用于：
- 支持人脂肪干细胞(ASC)生长
- 培养猪椎间盘(IVD)细胞和组织
- 培养软骨终板(CEP)细胞
Biochem/physiol Actions
- 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，可能会影响有丝分裂。
- 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了滋养培养细胞，可与血清转铁蛋白结合后使用。
- 硒：血清中的一种必需微量元素。
Preparation Note
使用不含酚红的Earle′平衡盐溶液（EBSS）制备。
Other Notes
含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、470 μg/m亚油酸、470 μg/ml 油酸和50 mg/ml牛血清白蛋白（100x 浓度）。每摩尔白蛋白含有 2 摩尔亚油酸和油酸。
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
高风险级别生物产品--人源产品
低风险生物材料
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| I2771-5ML | 04061833848678 |