type
Type IV-S
form
ammonium sulfate suspension
specific activity
10-40 units/mg protein (Bradford)
storage temp.
2-8°C
Quality Level
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General description
亮氨酸氨肽酶属于金属蛋白酶家族。
Application
猪肾来源微粒体亮氨酸氨肽酶用于从啮齿动物脑-C5 中分离脑啡肽酶(NEP),用于肽消化。
Biochem/physiol Actions
亮氨酸氨肽酶底物特异性广。能水解肽直至产生脯氨酸残基,不会对脯氨酸继续降解。
Physical form
3.5M (NH4)2SO4 溶液中的悬浮液,pH 7.7,含 10mM MgCl2
Other Notes
一个单位在37°C 下 pH 7.2 时每分钟将水解 1.0μ摩尔 L-亮氨酸-对硝基苯胺为 L -亮氨酸和对硝基苯胺(25°C 下可获得活性约为37°C 下的 40%)。以 L-亮氨酸对硝基苯胺为底物得到的活性是以 L-亮氨酸酰胺为底物得到的活性的 2-5 倍。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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We report a reaction-based strategy for the fluorogenic detection of protease activity. Based on the "covalent-assembly" probe design principle recently put forward by the Yang group for detection of Sarin related threats (J. Am. Chem. Soc., 2014, 136, 6594-6597), we
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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