N2787
Monoclonal Anti-Neurofilament 160 antibody produced in mouse
~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Neurofilament Medium Chain
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
RMO44, monoclonal
Application:
ARR, IHC, WB
Citations:
17
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RMO44, monoclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat, zebrafish
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable, microarray: suitable, western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
General description
Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.
Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.
Immunogen
purified mid-sized rat neurofilament (NF-M) subunit.
Application
Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- western blot analysis
- immunohistochemistry
- antibody labelling
Biochem/physiol Actions
Neurofilament 160 (NEFM) associates with the dopamine receptor.
Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Amanda L Jones et al.
Journal of neuroimmunology, 269(1-2), 68-75 (2014-03-19)
Schizophrenia is a severe debilitating brain disorder with a poorly understood aetiology. Among the diverse aetiological clues lies evidence for immune abnormalities in some individuals. The aim of this study was to investigate the frequency and specificity of autoantibodies directed
Nesreen Zoghoul Alsmadi et al.
Tissue engineering. Part C, Methods, 29(12), 547-557 (2023-09-24)
Traumatic injuries may result in the formation of soft tissue adhesions between peripheral nerves and surrounding soft tissue. These soft tissue adhesions lead to compression and ischemic stress within fascicles due to nonpliability of adhered scar tissue, and nerve tension
Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab
Liao ML, et al.
Journal of Neuroscience Research, 97(2), 202-214 (2019)
Neurofilaments at a glance
Yuan A, et al.
Journal of Cell Science, 125(14), 3257-3263 (2012)
An in vitro model for studying CNS white matter: functional properties and experimental approaches
Bijland S, et al.
F1000Research, 8(1) (2019)
相关内容
Datasheet
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持