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T6567

Trypsin Protease

Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas

别名:

Porcine Trypsin, Trypsin for Mass Spectropetry

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关于此项目

UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
232-650-8
MDL number:
MFCD01775455
Biological source:
Porcine pancreas
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产品名称

胰蛋白酶 来源于猪胰腺, Proteomics Grade, BioReagent, Dimethylated

biological source

Porcine pancreas

Quality Level

200

product line

BioReagent

solubility

1 mM HCl: soluble 1 mg/mL, clear, colorless

shipped in

wet ice

storage temp.

2-8°C

General description

胰蛋白酶是一种丝氨酸蛋白酶，通常在生物化学和生物学中用作重要的酶试剂。这种方法产生了适用于蛋白质组学研究的高度纯化的胰蛋白酶产物。蛋白质组学等级非常适合用于溶液和凝胶胰蛋白酶消化。胰蛋白酶是一种丝氨酸蛋白酶，存在于几种脊椎动物的消化系统中。

Application

猪胰脏胰蛋白酶被应用于以下应用中：
  • 凝胶内蛋白消化及MALDI-TOF质谱分析
  • 电喷雾电离质谱(ESI-MS)分析
  • L. 植物杆菌的表面蛋白质组分析
  • 凝胶过滤，超离心，旋转阴影电子显微镜
  • 质谱

Biochem/physiol Actions

由于胰蛋白酶具有高度特异性裂解能力，胰蛋白酶肽数量有限，因此胰蛋白酶通常用于蛋白质组学研究中的肽定位和蛋白质序列研究。它可特异性地水解精氨酸和赖氨酸残基羧基侧的肽键。该酶还具有酯酶和酰胺酶的活性。胰蛋白酶作为一种细胞培养工具。在工业上，它被用来水解致敏蛋白以生产低致敏牛奶。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000509346
0000510360
0000498514
0000498514
0000509346

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实验方案

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

商品

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Improving Glycoform Coverage for Mucin Preparations

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

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全球贸易项目编号

货号GTIN
T6567-1MG04061835491971
T6567-20UG04061835519132
T6567-5X20UG04061835575015