conjugate
agarose conjugate
type
Type III-ASCL
form
saline suspension
extent of labeling
~10 mg per mL
matrix
4% cross-linked beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~10 mg/mL binding capacity (peroxidase (P 8375))(pH 6.0), ~6 mg/mL binding capacity (yeast mannan)(pH 7.2)
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
Physical form
Suspension in 1.0 M NaCl containing 1 mM CaCl2, 1 mM MgCl2, 1 mM MnCl2 and 0.02% thimerosal
Preparation Note
One mL suspension yields approx. 0.5 ml gel.
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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