运输
dry ice
储存温度
−20°C
一般描述
The Caspase 6 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Val-Glu-Ile-Asp-7-amino-4-methyl coumarin (Ac-VEID-AMC) by caspase 6 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 440 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 6 activity in crude and purified preparations of caspase 6.
Complete kit contains:
The kit detects caspase 6 activity in crude and purified preparations of caspase 6.
Complete kit contains:
- purified caspase 6 for a positive control
- caspase 6 inhibitor
- AMC fluorescent standard
制备说明
The kit provides sufficient reagents for 100 tests of 100 μl in 96-well plates.
仅试剂盒组分
产品编号
说明
- Acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin Substrate .25 mL
- 7-Amino-4-methylcoumarin Standard 1 mL
- Caspase-6 5 μg
法规信息
新产品
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P Villa et al.
Trends in biochemical sciences, 22(10), 388-393 (1997-11-14)
Five years ago, little was known about mechanisms of apoptotic execution. Now, one class of cell-death gene, the cysteine and aspartases (caspases) has come under intensive study. This review discusses the two classes of caspases, the reasons why humans may
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