biological source
human blood
packaging
tube of 5 μg 01051619-DNA-5UG, pkg of vial of cells 01051619-1VL
growth mode
Suspension
karyotype
Tetraploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Internal control cell in telomere measurements performed by flow cytometry.
Biochem/physiol Actions
1301 is a sub-line of CCRF-CEM, Sigma catalogue no. 85112105. It has unusually long telomeres, useful in tests where telomere length is determined, where it can be used as control cells with known telomere length. This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation.
Human T-cell leukaemia
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 37°C; 5% CO2.
Other Notes
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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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