NTERA-2 clone D1 Cell Line human

Regulation of human embryonic differentiation, neuronal studies

Human Caucasian pluripotent embryonal carcinoma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12,13
D5S818: 9,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 18,19

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding 3-4 x 100,000 cells / cm²; harvest cells by scraping and maintain continuously at high density

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.