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Merck
CN

CARM-L1 TG3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

别名:

CARM, TG3

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UNSPSC代码:
41106514
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生物来源

rat lung

包装

tube of 5 μg 11120835-DNA-5UG
pkg of vial of cells 11120835-1VL

生长模式

Adherent

核型

Not specified

形态学

Epithelial and spindle shaped biphasic morphology

受体

Not specified

相关疾病

metastasis

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生化/生理作用

Not specified
Rat transplantable mesothelioma asbestos, primary tumour
The Me-series of esotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (Sigma product number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.

制备说明

DMEM (D6546)+ L-Glutamine (G7513) + 10% FBS / FCS (F2442).
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4 x 10,000 cells/cm2.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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