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UNSPSC Code:
41106514
Biological source:
rat lung
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial and spindle shaped biphasic morphology
biological source
rat lung
packaging
tube of 5 μg 11120835-DNA-5UG, pkg of vial of cells 11120835-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial and spindle shaped biphasic morphology
receptors
Not specified
relevant disease(s)
metastasis
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Biochem/physiol Actions
Not specified
Rat transplantable mesothelioma asbestos, primary tumour
The Me-series of esotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (Sigma product number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.
Preparation Note
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4 x 10,000 cells/cm2.
Other Notes
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法规信息
低风险生物材料
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