跳转至内容
Merck
CN

CPISO

Sigma-Aldrich

叶绿体分离试剂盒

isolation of intact chloroplasts from leaves

别名:

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

登录查看公司和协议定价

选择尺寸


关于此项目

UNSPSC代码:
12352200
NACRES:
NA.32
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

质量水平

技术

centrifugation: suitable

运输

wet ice

储存温度

2-8°C

一般描述

叶绿体分离试剂盒为从植物叶片中分离完整的叶绿体提供了有效的方法。该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用Percoll®分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。

应用

叶绿体分离试剂盒已采用菠菜、豌豆、生菜、卷心菜、莙达菜和烟草进行过测试。
在叶绿体细胞器研究中,叶绿体分离试剂盒可用于从植物叶片中分离叶绿体以便进行结构和功能研究。

特点和优势

  • 专门配制的缓冲液 - 采用易于执行的简单、成熟实验方案,节省时间
  • 可分离完整的叶绿体 - 适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程
  • 总细胞器蛋白质 - 适于进行功能性研究、代谢转运、蛋白质组谱分析和基因组应用
  • 规格方便 - 节省时间,减少浪费

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL

  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g化学品安全说明书

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea

  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mL化学品安全说明书

储存分类代码

10 - Combustible liquids

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Yuexia Wang et al.
BMC plant biology, 20(1), 96-96 (2020-03-07)
The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this
Yayi Tu et al.
PloS one, 10(8), e0136210-e0136210 (2015-08-27)
Potato virus Y (PVY) is an important plant virus and causes great losses every year. Viral infection often leads to abnormal chloroplasts. The first step of chloroplast division is the formation of FtsZ ring (Z-ring), and the placement of Z-ring
Juan Manuel Pérez-Ruiz et al.
The Plant cell, 18(9), 2356-2368 (2006-08-08)
One of the mechanisms plants have developed for chloroplast protection against oxidative damage involves a 2-Cys peroxiredoxin, which has been proposed to be reduced by ferredoxin and plastid thioredoxins, Trx x and CDSP32, the FTR/Trx pathway. We show that rice
Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
Jimena Cascales et al.
Life (Basel, Switzerland), 7(4) (2017-11-23)
The use of molecular markers with inadequate variation levels has resulted in poorly resolved phylogenetic relationships within Ilex. Focusing on southern South American and Asian species, we aimed at contributing informative plastid markers. Also, we intended to gain insights into

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持