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Merck
CN

CPISO

叶绿体分离试剂盒

isolation of intact chloroplasts from leaves

别名:

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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NACRES:
NA.32
UNSPSC Code:
12352200
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technique(s)

centrifugation: suitable

shipped in

wet ice

storage temp.

2-8°C

Quality Level

General description

叶绿体分离试剂盒为从植物叶片中分离完整的叶绿体提供了有效的方法。该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用Percoll®分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。

Application

叶绿体分离试剂盒已采用菠菜、豌豆、生菜、卷心菜、莙达菜和烟草进行过测试。
在叶绿体细胞器研究中,叶绿体分离试剂盒可用于从植物叶片中分离叶绿体以便进行结构和功能研究。

Features and Benefits

  • 专门配制的缓冲液 - 采用易于执行的简单、成熟实验方案,节省时间
  • 可分离完整的叶绿体 - 适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程
  • 总细胞器蛋白质 - 适于进行功能性研究、代谢转运、蛋白质组谱分析和基因组应用
  • 规格方便 - 节省时间,减少浪费

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL化学品安全说明书

  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g化学品安全说明书

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea化学品安全说明书

  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mL化学品安全说明书

存储类别

10 - Combustible liquids

法规信息

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Martha Sainz et al.
Physiologia plantarum, 140(1), 46-56 (2010-05-22)
Drought and heat stress have been studied extensively in plants, but most reports involve analysis of response to only one of these stresses. Studies in which both stresses were studied in combination have less commonly been reported. We report the
Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
Sarah Conte et al.
Plant physiology, 151(2), 559-573 (2009-08-14)
Widespread antibiotic resistance is a major public health concern, and plants represent an emerging antibiotic exposure route. Recent studies indicate that crop plants fertilized with antibiotic-laden animal manure accumulate antibiotics; however, the molecular mechanisms of antibiotic entry and subcellular partitioning
Yanzhong Luo et al.
Frontiers in plant science, 11, 613-613 (2020-06-13)
The transportation of proteins encoded by nuclear genes from plant cytosol to chloroplast is essential for chloroplast functions. Proteins that have a chloroplast transit peptide (cTP) are imported into chloroplasts via translocases on the outer and inner chloroplast envelope. How
The Complete Chloroplast Genome of 17 Individuals of Pest Species <I>Jacobaea vulgaris</I>: SNPs, Microsatellites and Barcoding Markers for Population and Phylogenetic Studies.
Doorduin, L., et al.
DNA Res., 1-13 (2011)

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