产品线
Duolink®
技术
proximity ligation assay: suitable
储存温度
20-25°C
应用
Experiments conducted using Duolink in situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
To perform a complete Duolink in situ experiment you will need two primary antibodies (IHC or IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA probes, one PLUS and one MINUS , your choice of Detection Reagents. Optional reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using the Duolink Image Tool.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF) or immunohistochemistry (IHC) assay to determine the optimal fixation, blocking, and titer conditions.
Duolink in situ reagents are suitable for use on fixed cells, cyt-spin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Find answers to commonly asked question on our Duolink FAQ page
To perform a complete Duolink in situ experiment you will need two primary antibodies (IHC or IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA probes, one PLUS and one MINUS , your choice of Detection Reagents. Optional reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using the Duolink Image Tool.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF) or immunohistochemistry (IHC) assay to determine the optimal fixation, blocking, and titer conditions.
Duolink in situ reagents are suitable for use on fixed cells, cyt-spin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Find answers to commonly asked question on our Duolink FAQ page
法律信息
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Asp. Tox. 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT RE 2 - STOT SE 3
靶器官
hearing organs, Respiratory system
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
73.4 °F - closed cup
闪点(°C)
23 °C - closed cup
法规信息
新产品
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相关内容
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
利用邻位连接技术对蛋白质互作、翻译后修饰和低表达蛋白进行检测、定量和成像应用。
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