EPI001
组蛋白乙酰转移酶 (HAT) 活性检测试剂盒
100 assays in 96 well plates
别名:
HAT Activity, Histone Acetyltransferase Activity
产品名称
组蛋白乙酰转移酶 (HAT) 活性检测试剂盒, 100 assays in 96 well plates
usage
100 assays in 96 well plates
shipped in
wet ice
storage temp.
−20°C
Features and Benefits
- 灵敏可靠的测定
- 利用比色法
- 样品类型：细胞和组织裂解液
- 种属反应性：哺乳动物
- 适用于独立测试或高通量分析和动力学研究
- 简便的过程：将核提取物或蛋白质样品与试剂混合、孵育并读取吸光度
- 方便的96孔微孔板规格
- 持续测量HAT活动；适用于动力学研究
- 无HDAC干扰；可直接使用核粗提物
General description
组蛋白乙酰转移酶（HAT）在细胞功能中发挥关键作用，它参与基因转录、分化和增殖的调控。HAT活性比色测定试剂盒提供了一种便捷的非放射性系统，可快速、灵敏地检测哺乳动物样品中的HAT活性。该试剂盒包含活性核提取物（NE）阳性对照、HAT辅因子、乙酰辅酶A以及所有下游辅因子和底物。活性HAT催化肽底物的乙酰化，释放出游离形式的辅因子，而辅因子是产生NADH所必需的辅酶。通过与可溶性四唑鎓染料反应，可使用分光光度法轻松检测到NADH。这种检测可以是连续性的，因此适用于动力学研究。该试剂盒为完整测定提供了所有试剂和简单的实验方案。
存储类别
12 - Non Combustible Liquids
法规信息
监管及禁止进口产品
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