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GE17-5166-01

Sigma-Aldrich

Mono Q® 5/50 GL

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NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

物料

PEEK column

描述

Ion Exchanger Type (value)

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pkg of 1 ea

参数

0.5 ml/min - 3.0 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
580 psi

柱床体积

1 mL

色谱柱内径

5.7 mm

基质

monodisperse porous polystyrene/divinyl benzene beads
polymeric

基质活性基团

ammonium ion, quaternary phase

平均零件尺寸

10 μm

cleaning

1-14

working range

2-12

分离技术

anion exchange

一般描述

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

特点和优势

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

储存及稳定性

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律信息

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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实验方案

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Affinity Chromatography in a Purification Strategy (CIPP)

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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