H4538
Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal
clone HDAC5-35, purified from hybridoma cell culture
别名:
Anti-HD5
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关于此项目
Conjugate:
unconjugated
Clone:
HDAC5-35, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
12
shipped in
dry ice
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HDAC5-35, monoclonal
form
buffered aqueous solution
mol wt
antigen ~124 kDa
species reactivity
rat, mouse, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 4-8 μg/mL using total cell extracts of NIH3T3 fibroblast cell or SW480 cell extract
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)
General description
Monoclonal Anti-Histone Deacetylase 5 (HDAC5) (mouse IgG1 isotype) is derived from the HDAC5-35 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Mammalian HDACs can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Histone deacetylase 5 (HDAC5) is a histone deacetylase, located on human chromosome 17q21.
Application
Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal has been used in:
- western blotting
- immunostaining
- enzyme-linked immunosorbent assay (ELISA)
- immunocytochemistry
- immunoprecipitation
Biochem/physiol Actions
Histone deacetylase 5 (HDAC5) promotes progression of the cell cycle, cell proliferation and apoptosis. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (Mef2) protein, thus repressing expression of MEF2 downstream genes. Overexpression of HDAC5 in different cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. Reduced expression of the protein is observed in colon cancer and acute myeloid leukemia.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
法规信息
低风险生物材料
常规特殊物品
此项目有
Histone Deacetylase 5 Is Overexpressed in Scleroderma Endothelial Cells and Impairs Angiogenesis via Repression of Proangiogenic Factors.
Tsou PS
Arthritis & Rheumatology (Hoboken, N.J.), 68(12), 2975-2985 (2016)
HDAC4 is required for inflammation-associated thermal hypersensitivity
Crow M, et al.
Faseb Journal (2015)
HDAC5 promotes optic nerve regeneration by activating the mTOR pathway
Pita-TW, et al.
Experimental Neurology, 317, 271-283 (2019)
HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells.
Peixoto P
Cell Death and Differentiation, 19(7), 1239-1252 (2012)
Megan Crow et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(8), 3370-3378 (2015-04-24)
Transcriptional alterations are characteristic of persistent pain states, but the key regulators remain elusive. HDAC4 is a transcriptional corepressor that has been linked to synaptic plasticity and neuronal excitability, mechanisms that may be involved in peripheral and central sensitization. Using
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