Anti-phospho-Insulin Receptor Substrate-1 (pSer³¹²) antibody produced in rabbit

Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. Phosphorylation of the Tyr¹¹⁷⁹ residue in the SH-1 negatively regulates insulin. IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. Estrogen receptors (ER) α and β have a strong affinity to IRS-1, an important interaction in breast cancers and medulloblastomas. The expression of IRS-1 in prostate cancer is associated with lower tumor cell invasiveness. Along with ER α, IRS-1 also modulates transcriptional activity involved in the development of breast and prostate cancer. The signalling pathway involving IGF-IR-IRS-1 in cells expressing JCV T-antigen has been reported to affect the proliferation, survival and DNA repair in the cells. Phosphorylation of IRS-1 at Ser312 and Ser616 results in the negative regulation of insulin signalling in humans
Anti-phospho-IRS-1 (pSer³¹²) specifically recognizes human IRS-1 (pSer³¹²). It may react with mouse and rat (100% homology) IRS-1.

synthetic phosphopeptide derived from the region of IRS-1 that contains serine 312. This sequence is 100% homologous with mouse and rat IRS-1.

Anti-phospho-IRS-1 (pSer³¹²) antibody may be used for detection by immunoblotting, in stimulated CHO cell line expressing human insulin receptor, at a working dilution of 1:1000.

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 containing 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.