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L3287

Escort ™ IV 转染试剂

Lipid reagent for transient and stable transfection of mammalian and insect cells.

别名:

Gene delivery

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关于此项目

NACRES:
NA.25
UNSPSC Code:
41106502
Form:
liquid (aqueous solution)
Grade:
Molecular Biology
Technique(s):
transfection: suitable
Concentration:
1 mg/mL
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grade

Molecular Biology

Quality Level

100

form

liquid (aqueous solution)

usage

 mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

相关类别

General description

Escort™ IV是由专利的聚阳离子脂质体和中性非转染脂质体构成的独特配方。这种脂质体构成的化合物可用于向不同类型的真核细胞转染核酸。

Application

适用于将核酸瞬时和稳定转染到培养的真核细胞中。 每 6 cm 细胞培养板使用约 4-16μL Escort™ IV 和 2μg DNA。方案优化提供了非常高效的转染。关于使用 Escort ™ IV 成功转染的细胞列表，请参见 转染试剂选择指南 。

Biochem/physiol Actions

在不存在血清条件下，将Escort™ IV与DNA混合，即可形成稳定的复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。

Features and Benefits

  • 适于稳定和瞬时转染
  • 针对广泛的细胞类型进行了优化
  • 低细胞毒性
  • 兼容含血清和不含血清的转染实验
  • 非常适于 Sf9, Sf21 和S2昆虫细胞

Other Notes

Escort™ IV 配方:
1 mg/mL溶于水的总脂质体

请注意，Escort™ IV 中使用的脂质体具体类别属于机密信息。

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

注意：不要冻存。

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCX8723
MKCW3393
MKCW6514
MKCV2499
MKCS9459

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R'ada Massarwa et al.
Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
Victoria J Hammond et al.
The Analyst, 133(1), 71-75 (2007-12-19)
Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
Melissa D Jordan et al.
Chemical senses, 34(5), 383-394 (2009-03-19)
Moths recognize a wide range of volatile compounds, which they use to locate mates, food sources, and oviposition sites. These compounds are recognized by odorant receptors (OR) located within the dendritic membrane of sensory neurons that extend into the lymph
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
Zev Bryant et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(3), 772-777 (2006-12-22)
Myosin VI supports movement toward the (-) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch of amino acids inserted between the converter domain and the lever arm was proposed to provide
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商品

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

细胞转染简介

转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。

实验方案

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

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相关内容

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

GenElute High Performance(HP) Plasmid Kits: Plasmid DNA from a Midiprep orMaxiprep in 30 Minutes

全球贸易项目编号

货号GTIN
L3287-SAMPLE04061833950982
L3287-1ML04061833950975

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