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MP0035

LookOut ® 支原体 PCR 检测试剂盒

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

别名:

PCR-based mycoplasma screening

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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quality

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

Quality Level

100

packaging

pkg of 1 kit

technique(s)

PCR: suitable

application(s)

detection
microbiology

compatibility

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

storage temp.

2-8°C

General description

该LookOut®支原体PCR检测试剂盒利用聚合酶链反应（PCR），这是因其在对细胞培养基中的支原体、无胆甾原体和尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。

Application

支原体PCR检测试剂盒视频实验方法
LookOut®支原体PCR检测试剂盒已用于支原体感染筛查。

Other Notes

该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3’-5’外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN6452
BCCM7998
BCCM5625
BCCL4114
BCCL4119

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Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

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Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

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实验方案

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

全球贸易项目编号

货号GTIN
MP0035-1KT04061834066835

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