等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
1000-5000 units/mL
运输
wet ice
储存温度
−20°C
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应用
Sau3AI is a DNA restriction enzyme that cuts DNA at the recognition sequence 5′-/GATC-3′ to generate DNA fragments containing 5′-cohesive termini.
生化/生理作用
Recognition sequence: 5′-/GATC-3′
Cutting results: A 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 20 minutes.
外形
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 250 mM NaCl, 5 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
其他说明
Comment: One unit cleaves approximately 98% of the substrate DNA.
Sau3A I activity is not blocked by dam methylation.
Sau3A I activity is not blocked by dam methylation.
Isoschizomers: MboI
Supplied with 10x Restriction Enzyme Buffer SA (B7531)
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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