等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
应用
ApaI is a restriction endonuclease used in molecular biology applications to cleave the recognition sequence 5′-GGGCC/C-3′ , generating DNA fragments with 3′-cohesive termini.
生化/生理作用
Recognition Sequence: 5′-GGGCC/C-3′
Cutting results: a 2-10-fold Apa I overdigestion of Ad-2 DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 15 minutes.
Cutting results: a 2-10-fold Apa I overdigestion of Ad-2 DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 15 minutes.
外形
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-Mercaptoethanol, 0.02% Polydocanol (v/v), 50% glycerol (v/v) at 4°C
其他说明
Comment: Ad-2 DNA is the substrate for the activity, cleavage, ligation, recleavage and overdigestion assays. Optimal activity is at 30 °C.
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
N Doan et al.
Journal of clinical microbiology, 38(8), 3043-3047 (2000-08-02)
Dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. Even though D. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism
J Seurinck et al.
Nucleic acids research, 11(13), 4409-4415 (1983-07-11)
A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA
G Basile et al.
Molecular biotechnology, 35(3), 253-261 (2007-07-27)
In this work we describe the production of site-specific biotinylated human myeloid differentiation factor 88 (MyD88). A vector containing a coding sequence for a peptide derived from the carboxyl terminus of the Klebsiella pneumoniae oxalacetate decarboxylase alpha subunit was used
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Diana Schenkwein et al.
Nucleic acids research, 41(5), e61-e61 (2013-01-01)
Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci.
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