S5174
SPARC from murine parietal yolk sac (PYS-2) cells
~80% (SDS-PAGE), lyophilized powder
别名:
BM-40, Osteonectin, Secreted protein acidic and rich in cysteine
生物来源
mouse (PYS-2 cells)
方案
~80% (SDS-PAGE)
表单
lyophilized powder
技术
cell culture | mammalian: suitable
表面覆盖度
4‑40 μg/cm2
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
mouse ... Sparc(20692)
应用
SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.
生化/生理作用
SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.
制备说明
This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.
其他说明
SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.
免责声明
The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
H Sage et al.
The Journal of cell biology, 109(1), 341-356 (1989-07-01)
SPARC (Secreted Protein Acidic and Rich in Cysteine) is a Ca+2-binding glycoprotein that is differentially associated with morphogenesis, remodeling, cellular migration, and proliferation. We show here that exogenous SPARC, added to cells in culture, was associated with profound changes in
G Chen et al.
British journal of cancer, 102(1), 188-195 (2009-11-19)
Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell-cell and cell-matrix interactions. In cancer, SPARC can be not only associated with a highly aggressive phenotype, but also acts as a tumour suppressor. The aim of this
T F Lane et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8(2), 163-173 (1994-02-01)
An extracellular matrix-associated glycoprotein expressed in a variety of tissues during embryogenesis and repair, SPARC contains modular domains that can function independently to bind cells and matrix components. Because SPARC can selectively disrupt cellular contacts with matrix and thereby effect
Clément Faye et al.
The Journal of biological chemistry, 284(33), 22041-22047 (2009-06-23)
Endostatin is a C-terminal proteolytic fragment of collagen XVIII that is localized in vascular basement membrane zones in various organs. It binds to heparin/heparan sulfate and to a number of proteins, but its molecular mechanisms of action are not fully
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