SAB4200087
Anti-Dicer antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-Dicer1, Anti-Endoribonuclease Dicer, Anti-HERNA, Anti-Helicase with RNase motif
产品名称
Anti-Dicer antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~218 kDa
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 3-6 μg/mL using HeLa cell lysates
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DICER1(23405)
mouse ... Dicer1(192119)
Application
Anti-Dicer antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescent staining
Biochem/physiol Actions
Anti-Dicer recognizes human Dicer.
Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.
Mutation in the Dicer gene causes malignancies.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.
Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Evolutionarily conserved roles of the dicer helicase domain in regulating RNA interference processing
Kidwell MA, et al.
The Journal of Biological Chemistry, 289(41), 28352-28362 (2014)
DICER regulates the expression of major satellite repeat transcripts and meiotic chromoso
Yadav RP, et al.
Nucleic Acids Research, 48(13), 7135-7153 (2020)
RNAi pathway participates in chromosome segregation in mammalian cells
Huang C, et al.
Cell discovery, 1, 15029-15029 (2015)
Molecular mechanisms of Dicer: endonuclease and enzymatic activity
Song MS and Rossi JJ
The Biochemical Journal, 474(10), 1603-1618 (2017)
The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development
Antoniou A, et al.
EMBO Reports, 19(3) (2018)
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