SAB4200193
Anti-Glyoxalase I antibody, Rat monoclonal
clone Clone 6F10, purified from hybridoma cell culture
别名:
Monoclonal Anti-Glyoxalase I antibody produced in rat
产品名称
Anti-Glyoxalase I antibody, Rat monoclonal, clone Clone 6F10, purified from hybridoma cell culture
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Clone 6F10, monoclonal
form
buffered aqueous solution
mol wt
~21 kDa
species reactivity
mouse, monkey, canine, rat, human
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
western blot: 0.12-0.25 μg/mL using A549, HeLa, SH-SY5Y, COS7 or MDCK cell extracts
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GLO1(2739)
mouse ... Glo1(109801)
rat ... Glo1(294320)
Application
Monoclonal Anti-Glyoxalase I antibody produced in rat has been used in immunoblotting. and immunocytochemistry.
Biochem/physiol Actions
The glyoxalase system plays a major role to detoxify α-ketoaldehydes, especially methylglyoxal (MG), that are endogenously formed as a by-product of the triosephosphate isomerase reaction during glycolysis. A member of this system, GLO1 catalyzes the isomerization of a hemithioacetal, comprised of a nonenzymatic adduct of MG and glutathione (GSH), to the corresponding α-d-hydroxyacid thioester and S-D-lactoylglutathione. Studies have suggested that GLO1 may be important for brain function since, GLO1 in the brain is involved in Alzheimer′s disease, autism, anxiety and the regulation of theta oscillations during sleep.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Glyoxalase I (rat IgG2b isotype) is derived from the hybridoma 6F10 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a mouse Glyoxalase I fusion protein. The glyoxalase system, which consists of glyoxalase I (GLO1), glyoxalase II, and a catalytic amount of reduced glutathione (GSH), is important part of cellular metabolism. GLO1 appears to be ubiquitously expressed in all mammalian cells, suggesting its biological importance.
Immunogen
mouse Glyoxalase I fusion protein
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
常规特殊物品
此项目有
Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease
Spanos C, et al.
Proteome Science, 16(1), 4-4 (2018)
Yusuke Nakadate et al.
Hybridoma (2005), 28(6), 447-450 (2009-12-23)
Glyoxalase I (GLO1) is a key enzyme that plays a role in the detoxification of methylglyoxal (MG), a toxic cellular metabolite produced during glycolysis. The present study reports on the preparation and properties of a monoclonal antibody (MAb) directed against
Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology
Pacal L, et al.
International Journal of Molecular Sciences, 19(5), 1517-1517 (2018)
Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice
Hovatta I, et al
Nature, 438(7068), 662-662 (2005)
Methylglyoxal: an emerging signaling molecule in plant abiotic stress responses and tolerance
Hoque TS, et al.
Frontiers in Plant Science, 7(20), 1341-1341 (2016)
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