SAB4200321
Anti-PTPRF (C-terminal) antibody produced in rabbit
IgG fraction of antiserum
别名:
Anti-LAR, Anti-Leukocyte antigen-related tyrosine phosphatase, Anti-Leukocyte common antigen-related tyrosine phosphatase, Anti-Tyrosine protein phosphatase receptor type F
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
6
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~150 kDa, antigen ~210 kDa, antigen ~85 kDa
species reactivity
human
technique(s)
immunoprecipitation (IP): 5-10 μL using lysates of HEK-293T cells over-expressing human PTPRF., western blot: 1:500-1:1000 using lysates of HEK-293T cells over-expressing human PTPRF.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PTPRF(5792)
mouse ... Ptprf(19268)
rat ... Ptprf(360406)
General description
PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule.
Protein tyrosine phosphatase, receptor type F (PTPRF) consists of noncovalently bound subunits designated as E (extracellular) and P (phosphatase) subunits that are derived from a precursor protein by proteolytic processing. The E subunit (150 kDa) has three immunoglobulin-like domains and eight fibronectin III-like domains, and the P subunit (85 kDa) has a short extracellular domain, a transmembrane segment and tandem cytoplasmic PTP catalytic domains.
Application
Anti-PTPRF (C-terminal) antibody produced in rabbit has been used in an enzyme based assay of protein tyrosine phosphatase 1 (PTP1).
The antibody may be used in several immunochemical techniques including immunoblotting (∼210, ∼150, and ∼85 kDa) and immunoprecipitation.
Biochem/physiol Actions
Protein tyrosine phosphatase, receptor type F (PTPRF) removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function. Insulin receptor, epidermal growth factor receptor (EGF) receptor, hepatocyte growth factor (HGF) receptor and rearranged during transfection (RET) have been reported as substrates of PTPRF.
The antibody recognizes both the extracellular and the cytoplasmic subunits of human PTPRF. Anti-PTPRF (C-terminal) antibody is specific for the P- subunit and the whole PTPRF protein from Human origin. Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Differential effects of leukocyte common antigen-related protein on biochemical and biological activities of RET-MEN2A and RET-MEN2B mutant proteins
Qiao S, et al.
The Journal of Biological Chemistry, 276(12), 9460-9467 (2001)
2, 5-DISUBSTITUTED OXADIAZOLE AND IT?S DERIVATIVE
INHIBIT PROTEIN TYROSINE PHOSPHATASE PTP1B, ENHANCING
INSULIN RECEPTOR PHOSPHORYLATION
Athare CL, et al.
World Journal of Pharmaceutical Research, 3(2), 2232-2247 (2014)
Mélanie J Chagnon et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 82(6), 664-675 (2005-01-28)
The protein tyrosine phosphatases (PTPs) have emerged as critical players in diverse cellular functions. The focus of this review is the leukocyte common antigen-related (LAR) subfamily of receptor PTPs (RPTPs). This subfamily is composed of three vertebrate homologs, LAR, RPTP-sigma
Robert A Mooney et al.
Current topics in medicinal chemistry, 3(7), 809-819 (2003-04-08)
The leukocyte common antigen-related protein, LAR, is a receptor-like protein tyrosine phosphatase (PTP) which has a wide tissue distribution. Post-translational processing cleaves the proprotein into two non-covalently associated subunits, an extracellular subunit resembling a cell adhesion molecule with three immunoglobulin-like
William J Polacheck et al.
Nature, 552(7684), 258-262 (2017-11-22)
The vascular barrier that separates blood from tissues is actively regulated by the endothelium and is essential for transport, inflammation, and haemostasis. Haemodynamic shear stress plays a critical role in maintaining endothelial barrier function, but how this occurs remains unknown.
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