SAB4200357
Anti-Profilin 1 antibody, Mouse monoclonal
clone Profilin 1-3, purified from hybridoma cell culture
别名:
Anti-PFN1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
Profilin 1-3, monoclonal
Application:
IP, WB
Species reactivity:
rat, bovine, human, mouse, canine
Citations:
5
Technique(s):
immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Profilin 1-3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~15 kDa
species reactivity
rat, bovine, human, mouse, canine
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PFN1(5216)
mouse ... Pfn1(18643)
rat ... Pfn1(64303)
Application
Monoclonal Anti-Profilin 1 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Profilin-1 (Pfn1) is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Profilin 1 is a potent regulator of actin filament dynamics. It is suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over expressed in breast cancer cells. Deletion of the profilin 1 gene leads to an embryonic lethal phenotype.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Profilin 1 (mouse IgG1 isotype) is derived from the hybridoma Profilin1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the C-terminus of human profiling 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin-1 (Pfn1), a major actin-regulatory protein is mapped to human chromosome17p13. It is the most ubiquitous and abundant protein and is highly expressed throughout development and adulthood in most tissues including brain.
Immunogen
synthetic peptide corresponding to a sequence located near the C-terminus of human profilin 1, conjugated to KLH. The corresponding sequence is identical in mouse and rat profilin 1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
PFN1 mutations are rare in Han Chinese populations with amyotrophic lateral sclerosis
Chen YP, et al.
Neurobiology of Aging, 34(7) (2013)
A balanced level of profilin-1 promotes stemness and tumor-initiating potential of breast cancer cells
Jiang C, et al.
Cell Cycle, 16(24) (2017)
Structure and functions of profilins
Krishnan K, et al.
Biophysical Reviews, 1(2), 71-71 (2009)
Sung Haeng Lee et al.
Molecules and cells, 29(4), 311-325 (2010-05-07)
The dynamic remolding of the actin cytoskeleton is a critical part of most cellular activities, and malfunction of cytoskeletal proteins results in various human diseases. The transition between two forms of actin, monomeric or G-actin and filamentous or F-actin, is
Profilin I is essential for cell survival and cell division in early mouse development
Witke W, et al.
Proceedings of the National Academy of Sciences of the USA, 98(7), 3832-3836 (2001)
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