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Merck
CN

T2413

Sigma-Aldrich

Trizma® 盐酸盐 溶液

1 M, pH 7.0

别名:

Tris 盐酸盐溶液

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关于此项目

MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
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等级

Molecular Biology

无菌性

0.2 μm filtered

表单

liquid

浓度

1 M

杂质

DNase, RNase, NICKase and protease, none detected

pH值(酸碱度)

7.0

应用

diagnostic assay manufacturing

储存温度

room temp

SMILES字符串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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应用

所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液,温度每降低 1°C,pH 增加约 0.03 个单位,浓度每稀释 10 倍,pH 降低 0.03-0.05 个单位。
对于精确应用,使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。

其他说明

用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Anthony G Boghdadi et al.
Nature communications, 12(1), 6906-6906 (2021-11-27)
Astrocytes play critical roles after brain injury, but their precise function is poorly defined. Utilizing single-nuclei transcriptomics to characterize astrocytes after ischemic stroke in the visual cortex of the marmoset monkey, we observed nearly complete segregation between stroke and control
Chromatin isolation by RNA purification (ChIRP).
Ci C Chu, Jeffrey J Quinn, Howard Y HY Chang
Journal of Visualized Experiments, 61 (2012)
Daniel Franjic et al.
Neuron (2021-11-20)
The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer's disease. To investigate neurogenic potential and cellular diversity, we profiled single-nucleus transcriptomes in five hippocampal-entorhinal subregions in humans, macaques, and pigs.
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Susana Mingote et al.
Bio-protocol, 7(1), e2090-e2090 (2017-01-05)
Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured

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