InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
SMILES string
Cl.NC(CO)(CO)CO
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
grade
Molecular Biology
sterility
0.2 μm filtered
form
liquid
concentration
1 M
impurities
DNase, RNase, NICKase and protease, none detected
pH
7.0
application(s)
diagnostic assay manufacturing
storage temp.
room temp
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Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
用 pH 可调的生物技术性能认证的 Trizma 碱在 18 兆欧水中制备并经过 0.2μm 过滤器过滤。
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
此项目有
Anthony G Boghdadi et al.
Nature communications, 12(1), 6906-6906 (2021-11-27)
Astrocytes play critical roles after brain injury, but their precise function is poorly defined. Utilizing single-nuclei transcriptomics to characterize astrocytes after ischemic stroke in the visual cortex of the marmoset monkey, we observed nearly complete segregation between stroke and control
Chromatin isolation by RNA purification (ChIRP).
Ci C Chu, Jeffrey J Quinn, Howard Y HY Chang
Journal of Visualized Experiments, 61 (2012)
Daniel Franjic et al.
Neuron (2021-11-20)
The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer's disease. To investigate neurogenic potential and cellular diversity, we profiled single-nucleus transcriptomes in five hippocampal-entorhinal subregions in humans, macaques, and pigs.
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Susana Mingote et al.
Bio-protocol, 7(1), e2090-e2090 (2017-01-05)
Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured
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