Anti-GBAS Antibody, clone 1H7 ZooMAb^® Rabbit Monoclonal

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1H7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Protein NipSnap homolog 2 (NIPSNAP2/GBAS). It targets an epitope within 20 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Protein NipSnap homolog 2 (NIPSNAP2/GBAS).

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected GBAS in Human kidney tissue sections.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GBAS in SH-SY5Y cells.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GBAS in Human skeletal muscle tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound GBAS peptide with a KD of 5.3 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Protein NipSnap homolog 2 (UniProt: O75323; also known as NipSnap2, Glioblastoma-amplified sequence, GBAS) is encoded by the NIPSNAP2 (also known as GBAS) gene (Gene ID: 2631) in human. NIPSNAP2/GBAS is a member of an evolutionarily conserved protein family that also includes NIPSNAP1, NIPSNAP3A, and NIPSNAP3B. It is localized to mitochondria and is reported to play a role in oxidative phosphorylation. It is widely expressed with most abundant expression observed in heart and skeletal muscle. It contains an N-terminal mitochondrial targeting sequence that is sufficient for its import into the mitochondria. It also contains an internal mitochondrial localization sequence, which is absent in NIPSNAP3A, and NIPSNAP3B, and is vital for the accumulation of NIPSNAP1 and NIPSNAP2 on the outer mitochondrial membrane (OMM) following mitochondrial damage. It is reported to act as an eat-me signal for damaged mitochondria and is involved in recruiting autophagy receptors and adaptors, as well as Atg8 to facilitate mitophagy. It also allows a sustained recruitment of SQSTM1-like receptors to ensure efficient mitophagy. Two isoforms of NIPSNAP2/GBAS have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Abudu, YP., et al. (2019). 15(10); 1845-1847; Abudu, YP., et al. (2019). Dev. Cell 49(4); 509-525).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.