Anti-NAXE/AIBP Antibody, clone 2E22 ZooMAb^® Rabbit Monoclonal

NAD(P)H-hydrate epimerase (UniProt: Q8NCW5; also known as EC:5.1.99.6, Apolipoprotein A-I-binding protein (AI-BP), NAD(P)HX epimerase (NAXE), YjeF N-terminal domain-containing protein 1 (YjeF_N1) is encoded by the NAXE (also known as AIBP, APOA1BP, YJEFN1 ) gene (Gene ID: 128240) in human. It belongs to the epimerase family, which catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H, converting it back into its functional form. Structurally, NAXE is a homodimer and it contains domains necessary for binding to NAD(P)H and for its epimerase activity. NAXE is predominantly localized in the mitochondria and is also found in secreted form in extracellular space. It is ubiquitously expressed in various tissues, with higher expression levels observed in metabolically active tissues such as the liver, heart, and kidney. Mutations in the NAXE gene have been linked to a rare autosomal recessive severe neurometabolic disorder characterized by early-onset progressive encephalopathy with brain edema and/or leukoencephalopathy-1 (PEBEL1). These mutations lead to a loss of function of the NAXE protein, resulting in the accumulation of damaged NAD(P)HX, which disrupts cellular redox homeostasis and contributes to the pathogenesis of the disease. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human NAXE/AIBP.

Quality Control Testing

Evaluated by Western Blotting in HEK-293T cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NAXE/AIBP in HEK-293T cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NAXE/AIBP in lysate from human liver tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NAXE/AIBP in human kidney tissue sections and human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound NAXE/AIBP peptide with at least ten million-fold (10,000,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2E22 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects NAXE/AIBP. It targets an epitope within 17 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.